Optical Mapping of Langendorff-perfused Rat Hearts
نویسندگان
چکیده
منابع مشابه
Optical mapping of Langendorff-perfused rat hearts.
Optical mapping of the cardiac surface with voltage-sensitive fluorescent dyes has become an important tool to investigate electrical excitation in experimental models that range in scale from cell cultures to whole-organs([1, 2]). Using state-of-the-art optical imaging systems, generation and propagation of action potentials during normal cardiac rhythm or throughout initiation and maintenance...
متن کاملVoltage-sensitive dye mapping in Langendorff-perfused rat hearts.
An imaging system suitable for recordings from Langendorff-perfused rat hearts using the voltage-sensitive dye 4-[beta-[2-(di-n-butylamino)-6-naphthyl]vinyl]pyridinium (di-4-ANEPPS) has been developed. Conduction velocity was measured under hyper- and hypokalemic conditions, as well as at physiological and reduced temperature. Elevation of extracellular [K(+)] to 9 mM from 5.9 mM caused a slowi...
متن کاملRegional ischemia in hypertrophic Langendorff-perfused rat hearts.
Myocardial hypertrophy decreases the muscle mass-to-vascularization ratio, thereby changing myocardial perfusion. The effect of these changes on myocardial oxygenation in hypertrophic Langendorff-perfused rat hearts was measured using epimyocardial NADH videofluorimetry, whereby ischemic myocardium displays a high fluorescence intensity. Hypertrophic hearts, in contrast to control hearts, devel...
متن کاملMultiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
Optical imaging and fluorescent probes have significantly advanced research methodology in the field of cardiac electrophysiology in ways that could not have been accomplished by other approaches(1). With the use of the calcium- and voltage-sensitive dyes, optical mapping allows measurement of transmembrane action potentials and calcium transients with high spatial resolution without the physic...
متن کاملOptical mapping of Langendorff-perfused human hearts: establishing a model for the study of ventricular fibrillation in humans.
Our objective was to establish a novel model for the study of ventricular fibrillation (VF) in humans. We adopted the established techniques of optical mapping to human ventricles for the first time to determine whether human VF is the result of wave breaks and singularity point formation and is maintained by high-frequency rotors and fibrillatory conduction. We describe the technique of acquir...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2009
ISSN: 1940-087X
DOI: 10.3791/1138